Colloidal Silver nutritional products from Survival Enterprises

Hawkeye-Jensen, 
Inc. 4408-B Pottsville Pike Reading, Pennsylvania 19605

TEST SAMPLES:
1ppm Silver Water® solution
3ppm Silver Water® solution
20ppm Silver Water® solution
1.0% Germaben II solution

TEST:
24-hour reduction test

TEST ORGANISMS:

Esherichia Coli ATCC 13047

Pseudomonas Aeruginose ATCC 27853

Staphylococcus Aurous ATCC 6538

Candida Albicans ATCC 14053

Aspergillus niger ATCC 16404

OBJECTIVE:
To determine if any of the above test samples has the ability to be substantially
detrimental to a significant population of known organisms.

PROCEDURE:
Twenty milliliters (ml) of sample were transferred to each of five sterile, capped sample cups.
Each cup was inoculated with a calculated inoculum of approximately 1 x 10 of each of the
given microbial suspensions and mixed. Samples were taken and plated at 24 hours after inoculation.

RESULTS:

	3ppm	10ppm	20ppm	1.0% Germaben II
E.Coli	130	<10	50	<10
Aeruginosa	50	<10	10	<10
Staph.Aureus	4,000	750	370	2,000
C.Albicans	50	80	70	<10

HOW TO READ THIS LAB TEST:

The results show and compare how much each test organism was
reduced in each product (3,10, 20 and 1.0%) in a 24-hour period.

Starting with approximately a 750,000 population of each organism, the 3ppm reduced the E.Coli 130
times, the l0ppm less than ten times, the 20ppm 50 times and the 1.0% Germaben II less than 10.

Test Organisms:

Group	Organisms Used
Ferm. Gram-neg	Enterbacter Cloacae Eacherichia Coli	ATCC 13047 ATCC11775
Non-form.Gram-negs	Pseudomonas Aeruginosa Burkholderia Cepacia	ATCC 27853 ATCC25415
Gram Pos. Cocci	Staphylococcus Aureus	ATCC 6538
Yeasts	Candida Albicans	ATCC 14053
Molds	Aspergillus Niger	ATCC 18404

Culture Maintenance:

Culture	Maintenance Agar	Storage	Transfer Options
Bacteria	Trypticase Soy	Refrigeration	Monthly
Yeasts	Bactrol Disks	Refrigeration	As needed
Molds	Sabouraud Dextrose	Refrigeration	Monthly

Diluents/Recovery,Media

Diluents	Agars
Lotheen broth	Lotheen agar (bacteria, yeasts)
	Sabouraud Dextrose (molds)

Inoculum Preps:

Culture	Incubation
Bactaris	30-35 C/24-48 hrs.
Yeasts	same
Molds	20-25 C/7-14 days

Procedure:

1. At least 20 g of product is inoculated with microbial suspension, so as to give 10 bacteria or 10 yeasts or molds per gram of product.

2. Using minimum sample size of 1 g. prepare decimal dilutions, and determine plate count/g of sample, by pour or surface plating methods.

3. Read plates after 2-3 days @ 30-35 C (bacteria/yeasts), and 3-7 days @ 20-25 C (molds).

4. Resample @ 1,2,3,7,14,28 days at ambient temp.

5. Rechallenge if specified.

Interpretation:

At least 99.9% reduction (veg. Bacteria)/90.0% reduction (molds) within 7 days. Ref: CTFA 1993 Sect. 13 pp 1-2

Challenge testing for the Burn Cream with Colloidal silver was begun on 10/22/97 for the yeast and bacteria groups (excluding Gram positive, which has not been started). And 10/30/97 for the mold group.

The yeast on bacteria groups were reduced 99.99% by day 1 and continued through day 7. The mold had a lesser percent reduction on day one however further testing must be done to make substantial claim.

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"These statements have not been evaluated by the FDA.
This product is not intended to diagnose, cure, prevent or treat any diseases."
The above is a Government ORDERED statement.
It is NOT based in either reality or sanity.
Just like our Government.

In a landmark decision on Friday, Jan. 15, 1999, the US Court of Appeals for the District of Columbia ruled that the health claim rules imposed by the FDA unconstitutional and in violation of the Administrative Procedure Act. The court instructed the FDA to define "significant scientific agreement" for health claims on dietary supplement labels, and instructed the FDA to allow the use of disclaimers on labels rather than to suppress these claims outright. The court further held that four FDA Final rules (prohibiting certain nutrient disease relationship claims) invalid under the first Amendment to the Constitution.