Hawkeye-Jensen,
Inc. 4408-B Pottsville Pike Reading, Pennsylvania 19605
TEST
SAMPLES: 1ppm
Silver Water® solution
3ppm
Silver Water®
solution
20ppm
Silver Water® solution
1.0% Germaben II solution
TEST:
24-hour reduction test
TEST ORGANISMS:
| Esherichia Coli | ATCC 13047
|
| Pseudomonas Aeruginose | ATCC
27853 |
| Staphylococcus Aurous | ATCC
6538 |
| Candida Albicans | ATCC
14053 |
| Aspergillus niger | ATCC
16404 |
OBJECTIVE:
To determine if
any of the above test samples has the ability to be substantially
detrimental
to a significant population of known organisms.
PROCEDURE:
Twenty milliliters (ml) of sample were transferred to each of five sterile,
capped sample cups.
Each cup was inoculated with a calculated inoculum of
approximately 1 x 10 of each of the
given microbial suspensions and mixed.
Samples were taken and plated at 24 hours after inoculation.
RESULTS:
| 3ppm | 10ppm | 20ppm | 1.0%
Germaben II |
| E.Coli | 130 | <10 | 50 | <10 |
| Aeruginosa | 50 | <10 | 10 | <10 |
| Staph.Aureus | 4,000 | 750 | 370 | 2,000 |
| C.Albicans | 50 | 80 | 70 | <10 |
HOW TO READ THIS LAB TEST:
The
results show and compare how much each test organism was
reduced in each product
(3,10, 20 and 1.0%) in a 24-hour period.
Starting with approximately
a 750,000 population of each organism, the 3ppm reduced the E.Coli 130
times, the l0ppm less than ten times, the 20ppm 50 times and the
1.0% Germaben II less than 10.
Test Organisms:
| Group | Organisms
Used | |
| Ferm. Gram-neg | Enterbacter
Cloacae Eacherichia Coli | ATCC 13047 ATCC11775 |
| Non-form.Gram-negs | Pseudomonas Aeruginosa Burkholderia Cepacia | ATCC
27853 ATCC25415 |
| Gram Pos. Cocci | Staphylococcus
Aureus | ATCC 6538 |
| Yeasts | Candida
Albicans | ATCC 14053 |
| Molds | Aspergillus
Niger | ATCC 18404 |
Culture
Maintenance:
| Culture | Maintenance Agar | Storage | Transfer
Options |
| Bacteria | Trypticase Soy | Refrigeration | Monthly |
| Yeasts | Bactrol Disks | Refrigeration | As needed |
| Molds | Sabouraud Dextrose | Refrigeration | Monthly |
Diluents/Recovery,Media
| Diluents | Agars |
| Lotheen broth | Lotheen agar (bacteria, yeasts) |
| Sabouraud Dextrose (molds) |
Inoculum
Preps:
| Culture | Incubation |
| Bactaris | 30-35 C/24-48 hrs. |
| Yeasts | same |
| Molds | 20-25 C/7-14 days |
Procedure:
1. At least 20 g of product
is inoculated with microbial suspension, so as to give 10 bacteria or 10 yeasts
or molds per gram of product.
2. Using minimum sample size of
1 g. prepare decimal dilutions, and determine plate count/g of sample, by pour
or surface plating methods.
3. Read plates after 2-3 days @ 30-35
C (bacteria/yeasts), and 3-7 days @ 20-25 C (molds).
4. Resample
@ 1,2,3,7,14,28 days at ambient temp.
5. Rechallenge if specified.
Interpretation:
At least 99.9% reduction (veg.
Bacteria)/90.0% reduction (molds) within 7 days. Ref: CTFA 1993 Sect. 13 pp 1-2
Challenge testing for the Burn Cream with Colloidal silver was
begun on 10/22/97 for the yeast and bacteria groups (excluding Gram positive,
which has not been started). And 10/30/97 for the mold group.
The
yeast on bacteria groups were reduced 99.99% by day 1 and continued through day
7. The mold had a lesser percent reduction on day one however further testing
must be done to make substantial claim.
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