Hawkeye-Jensen,Inc. 4408-B Pottsville Pike Reading, Pennsylvania 19605 TEST SAMPLES: 1ppm Silver Water® solution 3ppm Silver Water® solution 20ppm Silver Water® solution 1.0% Germaben II solution TEST: 24-hour reduction test TEST ORGANISMS: Esherichia Coli   ATCC 13047 Pseudomonas Aeruginose  ATCC 27853 Staphylococcus Aurous  ATCC 6538 Candida Albicans ATCC 14053 Aspergillus niger   ATCC 16404 OBJECTIVE: To determine if any of the above test samples has the ability to be substantially detrimental to a significant population of known organisms. PROCEDURE: Twenty milliliters (ml) of sample were transferred to each of five sterile, capped sample cups. Each cup was inoculated with a calculated inoculum of approximately 1 x 10 of each of the given microbial suspensions and mixed. Samples were taken and plated at 24 hours after inoculation. RESULTS:   3ppm 10ppm   20ppm 1.0% Germaben II E.Coli  130  <10 50 <10 Aeruginosa 50 <10 10 <10 Staph.Aureus 4,000 750 370 2,000 C.Albicans 50  80  70 <10 HOW TO READ THIS LAB TEST: The results show and compare how much each test organism was reduced in each product (3,10, 20 and 1.0%) in a 24-hour period. Starting with approximately a 750,000 population of each organism, the 3ppm reduced the E.Coli 130 times, the l0ppm less than ten times, the 20ppm 50 times and the 1.0% Germaben II less than 10.   Test Organisms: Group Organisms Used Ferm. Gram-neg  Enterbacter Cloacae Eacherichia Coli ATCC 13047 ATCC11775 Non-form.Gram-negs Pseudomonas Aeruginosa Burkholderia Cepacia ATCC 27853 ATCC25415 Gram Pos. Cocci Staphylococcus Aureus  ATCC 6538 Yeasts  Candida Albicans  ATCC 14053 Molds Aspergillus Niger ATCC 18404   Culture Maintenance:   Culture Maintenance Agar Storage  Transfer Options  Bacteria Trypticase Soy Refrigeration Monthly Yeasts Bactrol Disks Refrigeration As needed  Molds Sabouraud Dextrose Refrigeration Monthly   Diluents/Recovery,Media   Diluents Agars Lotheen broth Lotheen agar (bacteria, yeasts) Sabouraud Dextrose (molds) Inoculum Preps:   Culture  Incubation  Bactaris 30-35 C/24-48 hrs.  Yeasts same Molds 20-25 C/7-14 days   Procedure: 1. At least 20 g of product is inoculated with microbial suspension, so as to give 10 bacteria or 10 yeasts or molds per gram of product. 2. Using minimum sample size of 1 g. prepare decimal dilutions, and determine plate count/g of sample, by pour or surface plating methods. 3. Read plates after 2-3 days @ 30-35 C (bacteria/yeasts), and 3-7 days @ 20-25 C (molds). 4. Resample @ 1,2,3,7,14,28 days at ambient temp. 5. Rechallenge if specified. Interpretation: At least 99.9% reduction (veg. Bacteria)/90.0% reduction (molds) within 7 days. Ref: CTFA 1993 Sect. 13 pp 1-2 Challenge testing for the Burn Cream with Colloidal silver was begun on 10/22/97 for the yeast and bacteria groups (excluding Gram positive, which has not been started). And 10/30/97 for the mold group. The yeast on bacteria groups were reduced 99.99% by day 1 and continued through day 7. The mold had a lesser percent reduction on day one however further testing must be done to make substantial claim.   Back to Colloidal Silver page. Go to main Nutritionals page. Question? Email us!   "These statements have not been evaluated by the FDA. This product is not intended to diagnose, cure, prevent or treat any diseases." The above is a Government ORDERED statement. It is NOT based in either reality or sanity. Just like our Government. In a landmark decision on Friday, Jan. 15, 1999, the US Court of Appeals for the District of Columbia ruled that the health claim rules imposed by the FDA unconstitutional and in violation of the Administrative Procedure Act. The court instructed the FDA to define "significant scientific agreement" for health claims on dietary supplement labels, and instructed the FDA to allow the use of disclaimers on labels rather than to suppress these claims outright. The court further held that four FDA Final rules (prohibiting certain nutrient disease relationship claims) invalid under the first Amendment to the Constitution.